Nat. Commun.｜机制驱动定向挖掘新型广谱P450除草剂抗性基因

TL;DR - A Nature Communications study used mechanism-guided enzyme discovery and engineering to create a bacterial P450 variant that broadly degrades carboxyl-containing herbicides and confers herbicide resistance to transgenic rice.

• P450 BSβ-F46A degraded 10 herbicides, reaching 86% conversion for fluchloraminopyr and nearly 100% for 2,4-DB.
• The F46A mutation widened the substrate channel and lowered the calculated entry barrier from about 30 to 13 kcal mol⁻¹.
• The enzyme performs sequential ether-bond cleavage and aromatic-ring hydroxylation through a carboxylate-anchoring mechanism.
• A chloroplast-targeted P450 BSβ-F46A–CPR module gave rice substantial resistance to fluchloraminopyr in laboratory and spray tests.