Spatio-DARLIN maps cell state and clonal history in intact mouse tissues

TL;DR - Spatio-DARLIN is a method that pairs high-diversity genetic lineage tracing with spatial transcriptomics, letting researchers simultaneously read out cell lineage, gene expression, and physical location within a single intact mouse tissue section. This matters because it links clonal history to spatial context, illuminating how tissues develop and maintain themselves.

• Combines a high-diversity DARLIN lineage-tracing system with spatial transcriptomics in the same tissue slice, capturing clonal identity, transcriptome, and location together.
• Reported to reliably recover thousands of clones, indicating high barcode diversity and clone-resolution power.
• Applied to mouse intestine and brain, showing clonal architecture maps onto tissue development and homeostasis.
• A methods paper (Nature Methods); the summary describes capabilities and applications rather than detailed quantitative benchmarks.