Nature：清华大学王海峰团队开发新型基因敲入技术，无需切断DNA双链，高效精准敲入大片段基因

TL;DR - A Nature paper introduces KNIT editing, a CRISPR method that precisely inserts kilobase-scale DNA without double-strand breaks. It could enable safer genetic-disease treatments and CAR-T engineering.

• Combines Cas9(D10A) nickase with an mSA–biotin system that recruits donor DNA to single-strand nick sites for HDR.
• Inserted 0.7–10.5 kb DNA fragments with efficiencies up to 89% while reducing indels, translocations, and off-target editing.
• Corrected LIPA-related disease cells and restored gene expression, achieving 64% insertion at the AAVS1 safe-harbor site.
• Inserted CD19 CAR into the TRAC locus at up to 40.6%, producing viable CAR-T cells with antitumor activity in vitro and in mice.