浙大×哈工大合作Nature：超分辨化学显微术，长时间看清活细胞中的“纳米世界”

TL;DR - A Nature paper introduces RIED, a reaction-luminescence-based computational imaging method that achieves roughly 100 nm super-resolution without excitation light. Its zero-phototoxicity bioluminescence mode enabled continuous live-cell imaging for up to 41 hours.

• RIED combines entropy weighting, spatiotemporal correlation, and sparse deconvolution to recover structure from low-photon luminescence events.
• The method supports electrochemiluminescence, chemiluminescence, and bioluminescence, achieving lateral resolutions of 97–117 nm.
• RIED-ECL delivered 3D and large-field imaging while lowering the cellular carcinoembryonic-antigen detection threshold eightfold versus fluorescence SACD.
• Long-duration RIED-BL imaging quantified mitochondrial dynamics and tracked two modes of intercellular mitochondrial transfer.