Nat Biotechnol | 魏迪明团队与合作者开发下一代高通量基因从头合成技术

TL;DR - A Nature Biotechnology paper introduces MOSAIC, a high-throughput DNA synthesis platform that replaces polymerase extension with programmable oligonucleotide self-assembly and host-cell repair. It enables difficult sequence synthesis and large-scale gene and mutant-library construction at substantially lower cost and labor.

• Reversible hybridization provides self-correction and self-sorting, supporting 100% GC, highly structured, repetitive, and other challenging sequences.
• MOSAIC can synthesize thousands of genes in parallel and construct controlled mutant libraries containing up to tens of millions of variants.
• A plastic-degrading enzyme evolution screen reportedly required two researchers, under three months, and less than RMB 100,000 at a scale traditionally demanding years and millions.
• The team plans MOSAIC 2.0 with 10–100× higher throughput and direct synthesis of gene clusters up to 100 kb.