All-optical multimodal mapping of single-cell-type-specific metabolic activities via REDCAT

TL;DR - REDCAT is an all-optical tissue-mapping method that combines stimulated Raman scattering microscopy with high-plex immunofluorescence to profile cell-type-specific molecular and metabolic features. It enables multimodal single-cell analysis while preserving spatial tissue context.

• Integrates label-free Raman-based imaging with multiplexed protein detection.
• Profiles proteins, lipids, nuclear metabolites, and redox metabolism at single-cell resolution.
• Links metabolic activity to specific cell types within intact tissue.
• Supports spatially resolved construction of richer cellular atlases.