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CFM: confinement force microscopy—a dynamic, precise and stable microconfiner for traction force microscopy in spatial confinement

Nature Methods Biomechanics Tools Fatemeh Abbasi, Katharina Rieck, Matthias Brandt, Maja Matis, Eva Kiermaier, Timo Betz 2026-09-14

TL;DR - Confinement force microscopy (CFM) is a microconfinement method for measuring traction forces from individual cells and cell aggregates in tissue-like spatial environments. It enables dynamic, precise, stable, and tunable confinement during force microscopy.

  • Measures cellular traction forces under controlled spatial confinement.
  • Supports both single cells and multicellular aggregates.
  • Mimics mechanical constraints found in tissue microenvironments.
  • The provided content does not report quantitative performance or experimental results.

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