Targeted genomic integration and rearrangement using prime assembly

TL;DR - Prime assembly is an RNA-guided genome-engineering method that integrates medium-to-large DNA sequences into human cells without double-strand breaks or dependence on cell-cycle progression. It broadens programmable editing to substantial insertions and large genomic rearrangements.

• Supports exon recoding and transgene insertion.
• Enables rearrangements spanning megabase-scale genomic regions.
• Avoiding double-strand breaks may reduce risks associated with break-dependent editing methods.
• Cell-cycle independence could expand the range of human cell types amenable to targeted genome engineering.