Targeted genomic integration and rearrangement using prime assembly
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TL;DR - Prime assembly is an RNA-guided genome-engineering method that integrates medium-to-large DNA sequences into human cells without double-strand breaks or dependence on cell-cycle progression. It broadens programmable editing to substantial insertions and large genomic rearrangements.
- Supports exon recoding and transgene insertion.
- Enables rearrangements spanning megabase-scale genomic regions.
- Avoiding double-strand breaks may reduce risks associated with break-dependent editing methods.
- Cell-cycle independence could expand the range of human cell types amenable to targeted genome engineering.
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Targeted genomic integration and rearrangement using prime assembly
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TL;DR - Prime assembly is an RNA-guided genome-engineering method that integrates medium-to-large DNA sequences into human cells without double-strand breaks or dependence on cell-cycle progression. It broadens programmable editing to substantial insertions and large genomic rearrangements.
- Supports exon recoding and transgene insertion.
- Enables rearrangements spanning megabase-scale genomic regions.
- Avoiding double-strand breaks may reduce risks associated with break-dependent editing methods.
- Cell-cycle independence could expand the range of human cell types amenable to targeted genome engineering.