Sequential reading of a stepwise-shortened peptide immobilized on nanopore

TL;DR - A nanopore-based “chop and measure” method sequences immobilized peptides at single-amino-acid resolution, potentially advancing precise protein analysis.

• Enzymatic digestion removes one N-terminal residue at a time.
• Nanopore measurements read each progressively shortened peptide.
• Repetitive N-terminus re-reading supports sequential residue identification.