A multimodal, all-optical platform for linking cell identity to metabolic function in intact tissues

TL;DR - REDCAT is an all-optical platform that connects cell identity with metabolic activity in intact tissues at single-cell and subcellular resolution. It could advance functional histopathology by revealing disease-associated metabolic changes and intratumoral heterogeneity.

• Integrates Raman imaging, autofluorescence, and high-plex immunofluorescence.
• Maps metabolic features alongside cellular identity without disrupting tissue structure.
• Resolved metabolic characteristics in normal lymph nodes and lipid-redox remodeling in lymphoma.
• Exposed metabolic heterogeneity within tumors, suggesting potential diagnostic and research applications.